XXV IUCr Satellite Workshop: Methods of Structural Biology

Joining CCP4/CCP-EM online workshop and CMS workshop

Dates:
 August 9 – 10, 2021 (CCP4/CCP-EM)
August 13, 2021 (CMS workshop)

Venue: Online + Institute of Biotechnology of the Czech Academy of Sciences, Biocev Centre, Vestec near Prague

Aim:   Due to the low interest of participants, the CMS Workshop was transformed in one-day seminar with laboratory tour. The talks will be given by speakers originally invited to the satellite workshop. The seminar will be hybrid with for both speakers and participants: the seminar is happening in Biocev Center and it is broadcsted using Zoom at the same time. Some speakers will be present in person, and some online only (see programme below). The tour of CMS will be available for people present in BIOCEV Center.

The CMS workshop will provide training and hands on experience in selected structural biology techniques available at the Centre of Molecular Structure (CMS) at IBT. Introductory talks will be accompanied by more advanced insights into biophysics, protein crystallization, X-ray diffraction, small angle X-ray scattering and structural mass spectrometry. Sessions at instruments will focus on training in selected techniques.

            The CCP4/CCP-EM online workshop will focus on theory and practice in computational approaches in bio-crystallography and Cryo-EM single particle analysis.

Format: The CCP4/CCP-EM workshop will be organized online by the CCP4 and CCP-EM staff. The CMS workshop, organized by the CMS staff, will be one-day hybrid seminar with on-site tour of CMS.

There is a one-day break – August 11 – for travel of those who want to participate in both workshops.

Participation fee:

CCP4/CCP-EM – free.
CMS workshop - free

To apply: applications are closed.

The on-site attandence of the CMS workshop is availble to anyone. For online attandance, please use following link:

Contact to organizers:

Jan Dohnálek (CMS) – dohnalek at ibt.cas.cz
Magdalena Schneiderová (CMS) - magdalena.schneiderova at ibt.cas.cz
Jan Stránský (CMS) – jan.stransky at ibt.cas.cz
CCP4/CCP-EM - ccp4 -at- stfc.ac.uk

The CMS seminar is kindly sponsored by:

antonpaar_logo excillum_logo dectris_logo bruker_logo

CCP4/CCP-EM online workshop

Monday, August 9
09:00-09:45 CCP4 Cloud Setup and General Introduction
09:45-10:45 Data processing and reduction
10:45-11:00 Break
11:00-12:00 Experimental Phasing and Density Modification
12:00-13:00 Lunch break
13:00-13:45 Molecular Replacement
13:45-14:45 Model Building and Refinement
14:45-15:00 Break
15:00-15:30 Automated Model Building
15:30-16:00 Validation and Deposition
16:00-16:30 Break
16:30-17:30 Introduction to CryoEM (talk)
17:30-18:00 CCP-EM (practical setup)

Tuesday, August 10
09:00-10:30 Single particle reconstruction (demo + short practical)
10:30-10:45 Coffee Break
10:45-11:15 Reconstructed volumes - interpretation, sharpening, noise reduction (practical)
11:15-12:00 De novo model building (practical)
12:00-13:00 Lunch
13:00-14:00 Model refinement and validation (practical)
14:00-15:00 Wrap-up / free time

Technical information/organizationalfor CCP4/CCP-EM

Participants will require access to a Linux or MacOS machine with CCP4 and CCP-EM installed. Access to Zoom and Slack will also be required.

CMS Workshop

Friday, August 13

The talks are available both online and in lecture hall in Biocev
12:00-12:30 Arrival  
12:30-12:35 Welcome  
12:35-13:05 Crystallization of membrane proteins using lipidic systems Martin Caffrey (on-site)
13:05-13:35 Protein crystallography at XFELs
Arwen Pearson (remote)
13:35-14:00 Coffee break  
14:00-14:30 Advanced SAXS modeling techniques for biological macromolecules in solution Dima Molodenskyi (remote)
14:30-15:15 Structural Mass Spectrometry Julien Marcoux (on-site)
15:15-15:35 Spectroscopic methods to study biomolecular structure and dynamics Gustavo Fuertes Vives (on-site)
15:45- 17:00

Tours of CMS facilities

2x3 parallel tours (~30 min):

CF Structural Mass Spectrometry
CF Biophysical Methods
CF Crystallization of macromolecules & CF Diffraction techniques

Tour starting times: 15:45, 16:30
Meeting point: main recption foyer, below the lecture hall

 On-site only

All times are in CEST (UTC+2)

Confirmed speakers/tutors:

Martin Caffrey (Trinity College, Dublin); Julien Marcoux (CNRS, Toulouse); Dima Molodenskyi (EMBL, Hamburg); Arwen Pearson (Universität Hamburg); Tatsiana Charnavets (IBT); Jiří Pavlíček (IBT); Petr Pompach (IBT); Jan Stránský (IBT)