R&D services

Our laboratories offer a range of techniques for the production, characterisation and measurement of biological macromolecule samples as a service to external parties.

 

CONTACTS

Ing. Jan Dohnálek, Ph.D.

phone: 325 873 758

e-mail: jan.dohnalek@ibt.cas.cz

 

RNDr. Lubica Škultétyová, Ph.D.

phone: 325 873 780

e-mail: lubica.skultetyova@ibt.cas.cz

Pure proteins and their intermediates

Pure proteins and their intermediates

Finished plasmid DNA, sequencing results confirming cloning success. Bacterial pellets containing the desired protein. Purified protein sample.
Properties of biomolecules: size distribution, structure, stability and conformation

Properties of biomolecules: size distribution, structure, stability and conformation

Sample quality (aggregation), particle size and molecular weight estimate, ratio of components in the case of mixtures. Offers measurement of the particle size distribution in solution, particle oligomerization, aggregation.
Characterization of interactions of biomolecules

Characterization of interactions of biomolecules

Characterization of affinities of biomolecular interaction. Characterization of affinities and rates of interaction of biomolecules immobilized on the sensor chip with molecules in solution.
Spectrophotometric and luminescence study of biomolecules

Spectrophotometric and luminescence study of biomolecules

Sample characterization by the measurement of their absorbance and fluorescence. Characterization of samples in the infrared spectral region by measuring absorption frequencies of some specific chemical groups (amide bond in proteins).
Secondary structure of proteins and conformation of biomolecules

Secondary structure of proteins and conformation of biomolecules

Determining the secondary structure types in protein: approximate fractions - % of helix, sheet, turns, and random structures.
Mass spectrometry of biomolecules

Mass spectrometry of biomolecules

Identification of proteins in complex mixtures and their relative quantification. Determination of recombinantly expressed or isolated proteins. Characterization of protein modifications. Determination of protein-protein interaction areas and structural changes by hydrogen-deuterium exchange and chemical cross-linking in combination with mass spectrometry.
High-resolution 3D structure of (bio)molecules

High-resolution 3D structure of (bio)molecules

For the subsequent experimental determination of the 3D structure at atomic level by diffraction analysis, (using the home source or synchrotron). Single crystal X-ray diffraction. Measurement of diffraction data of molecular crystals.